Lou Charkoudian
Associate Professor of Chemistry
KINSC E214A
(610) 896-2994
lcharkou@haverford.edu
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Publications

Education

B.S., Haverford College 

Ph.D., Duke University

NIH Postdoctoral Scholar, Stanford University 

Research & Teaching

My research and teaching interests lie at the intersection of organic chemistry and biology.  I enjoy collaborating with undergraduate students in the classroom and laboratory to understand how nature drives chemical innovation, and how humans can leverage this information to discover environmentally responsible routes to novel therapeutic agents. 

Funding

I'm very grateful to the following funding agencies for believing in our work: National Institutes of Health (R15), National Science Foundation (CAREER and RCN), The Camille & Henry Dreyfus Foundation and Research Corporation for Science Advancement (Cottrell Scholars Award and Cottrell College Science Award).

Recent Publications (since 2013)

  1. Sulpizio, A., Crawford, C.E.W., Koweek, R.S., Charkoudian, L.K.* "Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways. J. Biol. Chem., 2021, Accpeted.
  2. Klein, J. G., Wu, Y., Kokona, B., Charkoudian, L.K.* "Widening the Bottleneck: Heterologous expression, purification, and characterization of Ktedonobacter racimifer minimal type II polyketide synthase in Escherichia coli. Bioorganic. Med. Chem., 2020, 28, 115686.
  3. Kautsar, S., Blin, K., Shaw, S., Navarro, J., Terlouw, B., van der Hooft, J., van Santen, J., Tracanna, V., Suarez, H., Pascal, V., Salem, N., Alanjary, M., Robinson, S., Lund, G., Epstein, S.C., Sisto, A.C., Charkoudian, L.K., Collemare, J., Lininton, R., Weber, T., Medema, M.H. "MiBIG 2.0: A repository for biosynthetic gene clusters of known function. Nuc. Acids Res., 2020, 48.
  4. Acheampong, K.K., Kokona, B., Braun, G.A., Jacobsen, D.R., Johnson, K.A., Charkoudian, L.K.* "Colorimetric assay reports on acyl carrier protein interactions."  Sci. Reports, 2019, 9, 14489.
  5. Epstein, S. C., Winesett, E. S., Huff, A., Londergan, C. H.,* Charkoudian, L. K.* "Tracking carrier protein motions using Raman spectroscopy." Nature Comm., 2019,10, 2227.
  6. Henry, M., Shorter, S., Charkoudian, L. K., Heemstra, J. M., Corwin, L, A.* "FAIL is not a four-letter word: A theorhetical framework for exploring student approaches to academic challenge and response to failure." CBE Life Sci. Ed. 2019, 18, ar1-rm1. 
  7. Gruele, A., Charkoudian, L. K., Cryle, M. J.* "Studying trans-acting enzymes that target carrier protein-bound amino acids during non-ribosomal peptide synthesis." Methods in Enzymology, 2019, 617, 114.
  8. Lopes, L. E.,Waldis, S. J., Terrell, S. M.,Lindgren, K. A.*, Charkoudian, L. K.* “Vibrant symbiosis: Achieving reciprocal science outreach through biological art.” PLoS Biology, 2018, 16, e300006.
  9. Rivas, M. A.,Courouble, V. C., Baker, M. C., Cookmeyer, D. L., Fiore, K. E., Frost, A. J., Godbe, K. N., Jordan, M. R., Krasnow, E. N., Mollo, A., Nawal, S., Ridings, S. T., Keisuke, S., Shroff, K. D., Studnitzer, B., Thiele, G. A. R., Sisto, A. C., Huff, A. R., Fairman, R., Beld, J., Kokona, B.*, Charkoudian, L. K.*“The Effect of Divalent Cations on the Thermostability of Type II Polyketide Synthase Acyl Carrier Proteins.” AIChE Journal, 2018, 64, 4308-4318.
  10. Epstein, S. C.,Charkoudian, L. K.*, Medema, M. H.* “A Standardized Workflow for Submitting Data to the Minimum Information about a Biosynthetic Gene Cluster (MIBiG) Repository: Prospects for Research-Based Educational Experiences.” Stand. Genomic Sci., 2018, 13, 16.
  11. Haas, K. L.,* Heemstra, J. M., Medema, M. H., Charkoudian, L. K.*“Collaborating with Undergraduates to Contribute to Biochemistry Community Resources.” Biochemistry,2018, 57, 383-389.
  12. Cookmeyer, D. L., Winesett, S. E., Kokona, B., Huff, A. R., Aliev, S., Bloch, N. B., Bulos, J. A., Evans, I. L., Farge, C. R., Godbe, K. N., Khromava, M., Konstantinovsky, D. M., Lafrance, A., Lamacki, A. J., Parry, R. C., Quinn, J. M., Thurston, A. M., Tsai, K. J. S., Mollo, A.,Cryle, M. J., Fairman, R.*, Charkoudian, L. K.* “ Uncovering Protein-Protein Interactions through a Team-based Undergraduate Biochemistry Course.” PLoS Biology, 2017, 15: e2003145.
  13. Mollo, A.,von Krusenstiern, A. N., Bulos, J. A., Ulrich, V., Akerfeldt, K., Cryle, M. J., Charkoudian, L. K.* “P450 Monooxygenase ComJ Catalyses Side Chain Phenolic Cross-linking During Complestatin Biosynthesis.” RSC Advances, 2017, 7, 35376-35384.
  14. Thiele, G. A. R.,Friedman, C. P., Tsai, K. J. S., Beld, J., Londergan, C. H.*, Charkoudian, L. K.*“Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase-Carrier Protein Crosslink”.  Biochemistry, 2017, 56, 2533 – 2536.
  15. Finzel, K.*, Beld, J., Burkart, M., Charkoudian, L. K.  “Utilizing Mechanistic Crosslinking Technology to Study Protein-Protein Interactions: An Experiment Designed for an Undergraduate Chemistry Lab.” J. Chem. Ed.2017, 94, 375-379.
  16. Kittiä, T., Mollo, A.,Charkoudian, L. K.*,Cryle, M. J.* “Have Substrate, will Travel: New Structural Data Reveals the Motion of Carrier Proteins in Non-Ribosomal Peptide Synthesis.”  Angew. Chem. Int. Ed.  2016,55, 9834 – 9840.
  17. Charkoudian, L. K.,Sampson, N. S., Kumar, K., Kritzer, J.* “Designing Convergent Chemistry Curricula.”  Nat. Chem. Biol., 2016, 12, 382 – 386.
  18. Fuga Li, Y., Tsai, K., Harvey, C., Ary, B., Berlew, E., Boehman, B., Findley, D., Friant, A., Gardner, C., Gould, M.,Ha, J.H.,Lilley, B., McKinstry, E., Nawal, S., Parry, R., Rothchild, K., Silbert, S., Tentilucci, M., Thurston, A., Wai, R., Yoon, Y., Aiyar, R., Medema, M. H., Hillenmeyer, M. E.,* and Charkoudian, L. K.* “Comprehensive Curation and Analysis of Fungal Biosynthetic Gene Clusters of Published Natural Products.” Fungal Genet. & Biol., 2016.  89, 18-28.
  19. Kokona, B., Winesett, E. S., von Krusenstiern, A. N., Cryle, M. J., Fairman, R.* Charkoudian, L. K.*“Probing the Selectivity of Beta-hydroxylation Reactions in Non-ribosomal Peptide Synthesis using Analytical Ultracentrifugation.” Anal. Biochem.  2016, 495, 42-51.
  20. Hillenmeyer, M. H.*, Borisova, G. V., Berlew, E. E., Charkoudian, L. K.* “Evolution of Chemical Diversity by Coordinated Gene Swaps in Type II Polyketide Gene Clusters.” Proc. Natl. Acad. Sci. 2015, 112, 13952 – 13957.
  21. Morlon, H., O’Connor, T., Bryant, J. A., Charkoudian, L. K., Docherty, K. M., Jones, E., Kembel, S., Green, J. L., Bohannan, B. J. M.   “The Biogeography of Putative Microbial Antibiotic Production.” PLoS One.  2015, 23, e0130659.
  22. Johnson, M. N. R., Londergan, C. H.*, Charkoudian, L. K.*“Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins using Vibrational Spectroscopy.”  J. Am. Chem. Soc. 2014, 136, 11240-11243.  (Article highlighted in C&EN News).

Want to learn more?

For more information about my teaching and research interests, please see my lab webpage!