Haverford Biology Instruments
To schedule instrument time:
- Check instrument availability
- Log into the 'Reserve Equipment' calendar
- Choose an available time slot
Contact George Neusch if you need help operating any instrumentation.
Instrument Scheduling
Reserve a specific time for the instruments below via Google Calendar.
- Compound Light Microscope (L004)
Reserve Equipment - Immunofluorescence Microscope A (L006)
Reserve Equipment - Immunofluorescence Microscope B (L006)
Reserve Equipment - Confocal Microscope (L007)
Reserve Equipment - SMZ 1500 Stereoscope (L006)
Reserve Equipment - SMZ 1500 Stereoscope (L007)
Reserve Equipment - Scanning Electron Microscope (SEM, L008)
Reserve Equipment - Transmission Electron Microscope (TEM, L009)
Reserve Equipment - FACS Calibur Flow Cytometer (E106)
Reserve Equipment - FACS Aria II Flow Cytometer (S211)
Reserve Equipment - Tecan SpectraFluor Plus (S114)
Reserve Equipment
Instruments and Resources
Microscopes
Compound Light Microscopes
Compound Light Microscope (L004)

This compound light microscope has a 100x oil immersion objective and is capable of phase contrast. It has a high resolution color camera operating on the SPOT Advanced Third Party Software.
Confocal Microscope (L007)

The confocal microscope is equiped with an epi-fluorescence attachment and a confocal scan-head allowing you to observe your samples in the scope as well as on the monitor. The scope itself has 5 opticl filters for viewing DAPI, GFP, TRITC, CY GFP, and Y GFP. The confocal has three laser options 405nm, 488nm, and 543nm with three detectors 515/30, 605/70. It has a motorized focus for creating 3D images using NIS-Elements Advanced Research (Ar) software.
Immunofluorescence Microscope A and B (L006)

The IMF scopes are equiped with 100x oil immersion objectives, black and white Photometrics high resolution cameras, and optical filters for viewing DAPI, GFP, and TRITC. They operate using NIS-Elements (F).
Stereomicroscopes
SMZ 1500 Stereoscope (L006)

The Department has two disecting microscopes with epi-fluorescence attachments and oblique coherent contrast. This scope has a 1x objective with a maximum magnification of 112.5x and a high resolution color camera. The other scope has a 1.6x objective with a maximum magnification of 180x. The operating software for both scopes is NIS-Elements Basic Research (Br). The camera driver is the DS-U3.
Inverted Compound Light Microscopes
Nikon TS100 Microscope (S114)

The Nikon TS100 has a 4x, 10x, 20x, and 40x objective and is capable of phasecontrast. It is equiped with a high resolution digital camera, the DS-Ri, and a fluorecence attachment with one optical filter for viewing DAPI. The operating software for this scope is NIS-Elements (F). The inverted micrscope provides a higher magnification than a stereoscope with a much larger working distance than a standard compound light microscope.
Nikon TE2000-S Microscope (S207A)

The TE2000-S has a maximum objective magnification of 60x with multiple optical filters including ND, D, GIF, and NCB. The fluorescence attachment contains one filter for viewing DAPI. The DS-Fi high resolution camera utilizes both the NIS-Elements Basic Research (Br) and (F).
Electron Microscopes
Scanning Electron Microscope (L008)

The JEOL JSM-6510 scanning electron microscope is equiped with an enviromental stage and capable of both high and low vacuum. This device also has a backscattered electron detector which is excellent for looking at biological samples at low vacuum
Transmission Electron Microscope (L009)

The JEOL JEM-1400 is capable of accelerating voltages of 40, 60, 80, 100, and 120kV. The maximum resolution is approximately 3.8nm. It employs a Gatan CCD Digital camera using Digital Micrograph imaging software.
Flow Cytometers
FACS Aria II Flow Cytometer (S211)

The FACS Aria II has three lasers and is capable of diquishing up to 9 colors. This cell sorter is able to sort up to four different cell types at a much faster rate than the FACS Calibur.
FACS Calibur Flow Cytometer (E106)

The FACS Calibur is a flow cytometer capable of distinguishing 4 colors and sorting up to four different cell types.

