Haverford Biology Instruments
To schedule instrument time:
- Log into the 'Reserve Equipment' calendar
- Choose an available time slot
Note: Instrument calendars may sync with your Haverford google calendar. To do this please send George Neusch your email address and the name of the instrument calendar you would like to sync. Then you will be able to stretch your time slot over multiple hours in a single day without choosing the slots individually. Please do not use the duplicate function in "edit event details." That function does not appear to sync properly with the calendar on this webpage.
Contact George Neusch if you need help operating any instrumentation.
Reserve a specific time for the instruments below via Google Calendar.
For use of the Cicular Dichroism machine please contact Rob fairman or Bash Kokona.
- Compound Light Microscope (L004)
- Immunofluorescence Microscope A (L006)
- Immunofluorescence Microscope B (L006)
- Confocal Microscope (L007)
- SMZ 1500 Stereoscope (L006)
- SMZ 1500 Stereoscope (L007)
- Scanning Electron Microscope (SEM, L008)
- Transmission Electron Microscope (TEM, L009)
- FACS Calibur Flow Cytometer (E106)
- FACS Aria II Flow Cytometer (S211)
- Tecan SpectraFluor Plus (S211)
- Tecan Infinite F200 Pro(S211)
For instructions on how to use the above instruments, log sheets, and other resources please click on the following link Instruction and Support
How is the Biology Department using the KINSC Makerspace?
The Makerspace is a new addition to the KINSC as of the start of the 2013-14 Fall Semester. It resides in E110 and is home to a Makerbot Replicator 2x 3D printer, a NextEngine 3D Scanner, and a ShopBot CNC Router. The Robotics Club frequents this space as a place to explore computer science, logic design, and automation through the endeavour of building a robotic car from scratch utilizing the 3D printer to form the chasis and an Arduino microcontroller to automate the vehicle. The space is also regularly utilized by the Biology Department for the development of research tools, such as a Rapid Iterative Negative Geotaxis (RING) assay chamber, or a fly anesthetization apparatus, both seen looping below.
Compound Light Microscopes
Compound Light Microscope (L004)
This compound light microscope has a 100x oil immersion objective and is capable of phase contrast. It has a high resolution color camera operating on the SPOT 5.1 Advanced.
Confocal Microscope (L007)
The confocal microscope is equipped with an epi-fluorescence attachment and a confocal scan-head allowing you to observe your samples in the scope as well as on the monitor. The scope itself has 5 optical filters for viewing DAPI, GFP, TRITC, CY GFP, and Y GFP. The confocal has three laser options 405nm, 488nm, and 543nm with three detectors 515/30, 605/70. It has a motorized focus for creating 3D images using NIS-Elements Advanced Research (Ar) software.
Immunofluorescence Microscope A and B (L006)
The IMF scopes are equipped with 100x oil immersion objectives, black and white Photometrics high resolution cameras, and optical filters for viewing DAPI, GFP, and TRITC. They operate using NIS-Elements (F 4.0).
SMZ 1500 Stereoscope (L006)
The Department has two dissecting microscopes with epi-fluorescence attachments and oblique coherent contrast. This scope has a 1x objective with a maximum magnification of 112.5x and a high resolution color camera. The other scope has a 1.6x objective with a maximum magnification of 180x. The operating software for both scopes is NIS-Elements Basic Research (Br 4.0). The camera driver is the DS-U3.
Inverted Compound Light Microscopes
Nikon TS100 Microscope (S114)
The Nikon TS100 has a 4x, 10x, 20x, and 40x objective and is capable of phasecontrast. It is equipped with a high resolution digital camera, the DS-Ri, and a fluorescence attachment with one optical filter for viewing DAPI. The operating software for this scope is NIS-Elements (F). The inverted microscope provides a higher magnification than a stereoscope with a much larger working distance than a standard compound light microscope.
Nikon TE2000-S Microscope (S207A)
The TE2000-S has a maximum objective magnification of 60x with multiple optical filters including ND, D, GIF, and NCB. The fluorescence attachment contains one filter for viewing DAPI. The DS-Fi high resolution camera utilizes both the NIS-Elements Basic Research (Br 3.0) and (F 2.0).
Scanning Electron Microscope (L008)
The JEOL JSM-6510 scanning electron microscope is equipped with an environmental stage and capable of both high and low vacuum. This device also has a backscattered electron detector which is excellent for looking at biological samples at low vacuum. We also have a Cryo Puck for freezing small and large tissue samples using liquid nitrogen and visualizing them under low vacuum.
Transmission Electron Microscope (L009)
The JEOL JEM-1400 is capable of accelerating voltages of 40, 60, 80, 100, and 120kV. The maximum resolution is approximately 3.8nm. It employs a Gatan CCD Digital camera using Digital Micrograph imaging software.
FACS Aria II Flow Cytometer (S211)
The FACS Aria II has three lasers and is capable of distinguishing up to 9 colors. This cell sorter is able to sort up to four different cell types at a much faster rate than the FACS Calibur.
FACS Calibur Flow Cytometer (E106)
The FACS Calibur is a flow cytometer capable of distinguishing 4 colors and sorting up to four different cell types.
For Instruction Support, click here