Haverford Biology Instruments
To schedule instrument time:
- Check instrument availability
- Log into the 'Reserve Equipment' calendar
- Choose an available time slot
Contact George Neusch if you need help operating any instrumentation.
Reserve a specific time for the instruments below via Google Calendar.
- Compound Light Microscope (L004)
- Immunofluorescence Microscope A (L006)
- Immunofluorescence Microscope B (L006)
- Confocal Microscope (L007)
- SMZ 1500 Stereoscope (L006)
- SMZ 1500 Stereoscope (L007)
- Scanning Electron Microscope (SEM, L008)
- Transmission Electron Microscope (TEM, L009)
- FACS Calibur Flow Cytometer (E106)
- FACS Aria II Flow Cytometer (S211)
Instruments and Resources
Compound Light Microscopes
Compound Light Microscope (L004)
This compound light microscope has a 100x oil immersion objective and is capable of phase contrast. It has a high resolution color camera operating on the SPOT Advanced Third Party Software.
Confocal Microscope (L007)
The confocal microscope is equipped with an epi-fluorescence attachment and a confocal scan-head allowing you to observe your samples in the scope as well as on the monitor. The scope itself has 5 optical filters for viewing DAPI, GFP, TRITC, CY GFP, and Y GFP. The confocal has three laser options 405nm, 488nm, and 543nm with three detectors 515/30, 605/70. It has a motorized focus for creating 3D images using NIS-Elements Advanced Research (Ar) software.
Immunofluorescence Microscope A and B (L006)
The IMF scopes are equipped with 100x oil immersion objectives, black and white Photometrics high resolution cameras, and optical filters for viewing DAPI, GFP, and TRITC. They operate using NIS-Elements (F).
SMZ 1500 Stereoscope (L006)
The Department has two dissecting microscopes with epi-fluorescence attachments and oblique coherent contrast. This scope has a 1x objective with a maximum magnification of 112.5x and a high resolution color camera. The other scope has a 1.6x objective with a maximum magnification of 180x. The operating software for both scopes is NIS-Elements Basic Research (Br). The camera driver is the DS-U3.
Inverted Compound Light Microscopes
Nikon TS100 Microscope (S114)
The Nikon TS100 has a 4x, 10x, 20x, and 40x objective and is capable of phase contrast. It is equipped with a high resolution digital camera, the DS-Ri, and a fluorescence attachment with one optical filter for viewing DAPI. The operating software for this scope is NIS-Elements (F). The inverted microscope provides a higher magnification than a stereoscope with a much larger working distance than a standard compound light microscope.
Nikon TE2000-S Microscope (S207A)
The TE2000-S has a maximum objective magnification of 60x with multiple optical filters including ND, D, GIF, and NCB. The fluorescence attachment contains one filter for viewing DAPI. The DS-Fi high resolution camera utilizes both the NIS-Elements Basic Research (Br) and (F).
Scanning Electron Microscope (L008)
The JEOL JSM-6510 scanning electron microscope is equipped with an environmental stage and capable of both high and low vacuum. This device also has a backscattered electron detector which is excellent for looking at biological samples at low vacuum
Transmission Electron Microscope (L009)
The JEOL JEM-1400 is capable of accelerating voltages of 40, 60, 80, 100, and 120kV. The maximum resolution is approximately 3.8nm. It employs a Gatan CCD Digital camera using Digital Micrograph imaging software.
FACS Aria II Flow Cytometer (S211)
The FACS Aria II has three lasers and is capable of distinguishing up to 9 colors. This cell sorter is able to sort up to four different cell types at a much faster rate than the FACS Calibur.
FACS Calibur Flow Cytometer (E106)
The FACS Calibur is a flow cytometer capable of distinguishing 4 colors and sorting up to four different cell types.