The research in our laboratory focuses on the changes in
gene expression which accompany the activation of B and T
We are in the process of developing a novel mRNA
differential display method which yields 5' end gene
fragments from differentially expressed genes, in contrast
to the 3' fragments generated by more classical
methodologies. (Liang and Pardee, 1992, Science 257,
967-71). Our technique should allow for fewer instances of
detected fragments which fall in the long 3' untranslated
regions of eucaryotic genes and better isolation of the full
cDNA from the small gene fragments.
A second line of investigation addresses the timingof the
onset of expression of murine survivin or TIAP, in B and T
lymphocytes, as a function of both time and the number of
cell divisions undergone post activation.
In addition, projects are currently ongoing in
collaboration with Prof. Jenni Punt. We are looking for
genes which are differentially expressed in thymocytes
stimulated under varying conditions. A second pilot project
is exploring the possibility of using retroviral
transduction to introduce genes of interest into activated B
and T cells.
Work conducted in collaboration with Dr. Charles Owen,
Thomas Jefferson University, is aimed at understanding those
changes in gene expression which accompany tumor adaptation
to growth at low pH.